服务热线
021-54479081
021-54461587
产品展示PRODUCTS
本产品仅用于科研,不用于临床
小鼠活化素A酶免试剂盒,(ACV-A)ELISA检测试剂盒,英文名:ACV-A ELISA Kit,英文缩写:ACV-A ,常见试剂盒规格:96T/48T,产品别名:小鼠活化素AELISA检测试剂盒,ACV-A 检测试剂盒,ACV-A 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠活化素A酶免试剂盒,(ACV-A)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质??捎孟窗寤窗寤蚴止は窗澹罢呦吹又柿拷虾?,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
Slit同源物3(Slit3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Slit Homolog 3 (Slit3)48T/96TY13685Rattus norvegicus (Rat)
精氨酰tRNA合成酶(RARS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Arginyl tRNA Synthetase (RARS)48T/96TY13686Homo sapiens (Human)
电碳酸氢钠协同转运蛋白4(NBC4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4)48T/96TY13687Homo sapiens (Human)
Ras相关C3肉毒菌毒素底物1(Rac1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)48T/96TY13688Rattus norvegicus (Rat)
脱氧核糖核酸酶Ⅰ(DNASE1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Deoxyribonuclease I (DNASE1)48T/96TY13689Bos taurus; Bovine (Cattle)
非受体型蛋白酪氨酸磷酸酶11(PTPN11)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11)48T/96TY13690Rattus norvegicus (Rat)
赖氨酰氧化酶样蛋白1(LOXL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lysyl Oxidase Like Protein 1 (LOXL1)48T/96TY13691Homo sapiens (Human)
儿茶酚-O-甲基转移酶(COMT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Catechol-O-Methyltransferase (COMT)48T/96TY13692Rattus norvegicus (Rat)
核糖体蛋白S6激酶β1(RPS6Kβ1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1)48T/96TY13693Homo sapiens (Human)
骨髓基质细胞抗原1(BST1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bone Marrow Stromal Cell Antigen 1 (BST1)48T/96TY13694Homo sapiens (Human)
Saitohin蛋白(STH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Saitohin (STH)48T/96TY13695Homo sapiens (Human)
腺苷A2b受体(ADORA2b)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Adenosine A2b Receptor (ADORA2b)48T/96TY13696Homo sapiens (Human)
鸟苷酸环化酶激活因子2B(GUCA2B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Guanylate Cyclase Activator 2B (GUCA2B)48T/96TY13697Homo sapiens (Human)
组织金属蛋白酶抑制因子1(TIMP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY13698Bos taurus; Bovine (Cattle)
饥饿素(GHRL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ghrelin (GHRL)48T/96TY13699Ovis aries; Ovine (Sheep)
生长分化因子11(GDF11)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Growth Differentiation Factor 11 (GDF11)48T/96TY13700Mus musculus (Mouse)
SPARC样蛋白1(SPARCL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for SPARC Like Protein 1 (SPARCL1)48T/96TY13701Rattus norvegicus (Rat)
防御素β112(DEFβ112)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Defensin Beta 112 (DEFb112)48T/96TY13702Homo sapiens (Human)
血管生成素样蛋白6(ANGPTL6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiopoietin Like Protein 6 (ANGPTL6)48T/96TY13703Rattus norvegicus (Rat)
IgG-Fc片段低亲和力受体Ⅲa(FcγR3A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fc Fragment Of IgG Low Affinity IIIa Receptor (FcgR3A)48T/96TY13704Homo sapiens (Human)
信号传导转录激活因子3(STAT3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Signal Transducer And Activator Of Transcription 3 (STAT3)48T/96TY13705Rattus norvegicus (Rat)
载脂蛋白C3(APOC3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein C3 (APOC3)48T/96TY13706Rattus norvegicus (Rat)
载脂蛋白C2(APOC2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein C2 (APOC2)48T/96TY13707Rattus norvegicus (Rat)
Janus激酶2(JAK2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Janus Kinase 2 (JAK2)48T/96TY13708Rattus norvegicus (Rat)
旁血小板溶蛋白(PPL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Periplakin (PPL)48T/96TY13709Homo sapiens (Human)
Engrailed同源框蛋白2(EN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Engrailed Homeobox Protein 2 (EN2)48T/96TY13710Homo sapiens (Human)
胶质细胞系来源神经营养因子受体α1(GFRα1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1)48T/96TY13711Homo sapiens (Human)
肿瘤坏死因子α诱导蛋白6(TNFαIP6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6)48T/96TY13712Mus musculus (Mouse)
多聚A特异性核糖核酸酶(PARN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Poly A Specific Ribonuclease (PARN)48T/96TY13713Homo sapiens (Human)
氯化物细胞内通道蛋白1(CLIC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chloride Intracellular Channel Protein 1 (CLIC1)48T/96TY13714Homo sapiens (Human)
醌型二氢蝶啶还原酶(QDPR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Quinoid Dihydropteridine Reductase (QDPR)48T/96TY13715Homo sapiens (Human)
肌酸转运蛋白(CRT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Creatine Transporter (CRT)48T/96TY13716Homo sapiens (Human)
蛋氨酸腺苷转移酶Ⅰα(MAT1α)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Methionine Adenosyltransferase I Alpha (MAT1a)48T/96TY13717Mus musculus (Mouse)
双氧化酶2(DUOX2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dual Oxidase 2 (DUOX2)48T/96TY13718Homo sapiens (Human)
双氧化酶2(DUOX2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dual Oxidase 2 (DUOX2)48T/96TY13719Rattus norvegicus (Rat)
组蛋白H1(H1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Histone H1 (H1)48T/96TY13720Homo sapiens (Human)
苯丙氨酸羟化酶(PAH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phenylalanine Hydroxylase (PAH)48T/96TY13721Homo sapiens (Human)
苏氨酰tRNA合成酶(TARS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Threonyl tRNA Synthetase (TARS)48T/96TY13722Homo sapiens (Human)
γ-谷氨酰水解酶(γGH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Gamma-Glutamyl Hydrolase (gGH)48T/96TY13723Homo sapiens (Human)
胃泌酸调节素(OXM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Oxyntomodulin (OXM)48T/96TY13724Homo sapiens (Human)
硒结合蛋白1(SELENBP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Selenium Binding Protein 1 (SELENBP1)48T/96TY13725Homo sapiens (Human)
细胞色素P450家族成员2E1(CYP2E1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cytochrome P450 2E1 (CYP2E1)48T/96TY13726Mus musculus (Mouse)
衰老关键蛋白2(FBLN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibulin 2 (FBLN2)48T/96TY13727Homo sapiens (Human)
单核细胞趋化蛋白1(MCP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)48T/96TY13728Sus scrofa; Porcine (Pig)
趋化因子C-X-C-基元受体5(CXCR5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chemokine C-X-C-Motif Receptor 5 (CXCR5)48T/96TY13729Homo sapiens (Human)
白介素13受体α2(IL13Rα2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 13 Receptor Alpha 2 (IL13Ra2)48T/96TY13730Mus musculus (Mouse)
层黏连蛋白α3(LAMα3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Laminin Alpha 3 (LAMa3)48T/96TY13731Homo sapiens (Human)
亮氨酸丰富重复激酶2(LRRK2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leucine Rich Repeat Kinase 2 (LRRK2)48T/96TY13732Homo sapiens (Human)
甘露糖受体C2(MRC2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mannose Receptor C Type 2 (MRC2)48T/96TY13733Homo sapiens (Human)
E1A激活基因阻遏子1(CREG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cellular Repressor Of E1A Stimulated Genes 1 (CREG1)48T/96TY13734Homo sapiens (Human)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。